Figure 4 | Scientific Reports

Figure 4

From: Functional characterization and related evolutionary implications of invertebrate gonadotropin-releasing hormone/corazonin in a well-established model species

Figure 4

Double-labeling of ly-GnRH/CRZ neurons in the anterior lobe of the right cerebral ganglion by IHC and nickel-lysine backfill. (a,b) represent the same section. The section after nickel backfill was visualized without (a) and with (b) IHC imaging. Red color: ly-GnRH/CRZ immunostaining; blue color: nuclei staining. Arrow represents a ly-GnRH/CRZ-ir neuron. Arrowhead indicates a backfilled neuron. The double-labeled neuron is labeled by both arrow and arrowhead. Scale bar = 25 µm.

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