Figure 5

Representative 5-HT (green) and ly-GnRH/CRZ (red) IHC of the preputium (a1–a3) and the heart atria (b1–b3). Axon fiber bundles containing both 5-HT and ly-GnRH/CRZ (arrowheads) can be seen on the longitudinal muscle of the preputium. On the surface of the heart atria muscle, axon fiber bundles containing only ly-GnRH/CRZ (arrows) or both 5-HT and ly-GnRH/CRZ (arrowheads) are present. Scale bars = 40 µm (a1–a3) and 10 µm (b1–b3). LM longitudinal muscle.