Figure 6

NK92MI cells were transfected with siRNA control, siEGR-1, siCD59, siCXCR7, siGAS1, and siRAMP3; 48 h later, doxorubicin (10 µM) was applied for 24 h (A). Dot plots are separated into four quadrants: Q1 (Annexin V-/PI + : cell death), Q2 (Annexin V + /PI + : late apoptotic cells), Q3 (Annexin V − /PI − : living cells), and Q4 (Annexin V + /PI- : early apoptotic cells). The percentage of apoptotic cells measured using flow cytometry (B). Values are presented as mean ± SEM (n = 3). P values were determined using Student’s t-test. *P < 0.05 versus siCTL siRNA, small interfering RNA.