Figure 1

The effects of storage conditions on urinary cell viability. Cells are stored at different conditions and stained with 7-AAD. (a) Positive staining control: Starved HK2 cells; (b) Urinary cells kept at 4 °C overnight; (c–e) Urinary cells stored at − 80 °C in DMEM, HBSS and PBS + 10% DMSO respectively. (f) is the relative quantitation of b–e.