Figure 2
Mutations in 12 PgABCA2 cDNA sequences from six Cry2Ab-resistant pink bollworm larvae from F2 progeny of CRISPR-R2 X APHIS-S single-pair families. Mutations in PgABCA2 cDNAs from CRISPR-R2 survivors from three single-pair families (1–3 clones from each of five individuals from families A, C, and J) relative to the susceptible strain APHIS-S (MG637361). Numbers to the right of the family name indicates individual and clone number, respectively (e.g., A.1.1 indicates Family A, Individual 1, Clone 1). Exon numbers are shown for APHIS-S. Location of sgRNAs 1–5 are shown as colored bars (sgRNA1, magenta; sgRNA2, cyan; sgRNA3, blue; sgRNA4, yellow; sgRNA5, red). Red triangles above bars indicate premature stop codons. Colors within bars show mutations: orange for insertions and deletions (indels), red for deletions, and green for insertions.
