Scientific Reports

Table 1 Mutations corresponding to sgRNA target sites in PgABCA2 gDNA in 20 Cry2Ab-resistant pink bollworm larvae from the G₄ of the CRISPR-R2 strain.

From: CRISPR-mediated mutations in the ABC transporter gene ABCA2 confer pink bollworm resistance to Bt toxin Cry2Ab

Individual #	sgRNA 1	sgRNA 2	sgRNA 3	sgRNA 4	sgRNA 5
1	Indel^a	0^b	0	0	0
2	Indel	0	0	0	0
3^c	0	0	0	0	0
4	0	0	0	Substitution^d	Deletion
5^c	0	0	0	0	0
6	Indel	0	0	0	0
7	Indel	0	0	0	Deletion
8	Indel	0	0	0	0
9^c	0	0	0	0	0
10^c	0	0	0	0	0
11	0	0	0	0	Deletion
12	Indel	0	0	0	0
13	0	0	0	0	Indel
14	Indel	0	0	0	nd^e
15	Indel	0	0	0	nd
16	Indel	0	0	0	0
17	Indel	0	0	0	0
18^c	0	0	0	0	0
19	Indel	0	0	0	0
20	Indel	nd	nd	nd	Indel

^aInsertion/deletion mutation.
^bWild-type, sequence identical to PgABCA2 from APHIS-S.
^cLacked mutations at all five sgRNA target sites.
^dSingle base pair substitution.
^eNot determined, unable to sequence the PCR product.

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