Figure 3
From: Tubulin binding potentially clears up Bortezomib and Carfilzomib differential neurotoxic effect
Neuron cultures viability after treatment with BTZ and CFZ. (A) Representative images of neuron cultures treated for 48 h with 2.8 nM BTZ or 3.2 nM CFZ or untreated (CTRL). Bar = 400 μm. White arrows indicate viable neurons. (B) Graph represents the percentage of viable neurons after treatment with BTZ 2.8 nM and CFZ 3.2 nM and are compared to untreated CTRL arbitrarily set to 100%. (C) Representative images of normal neurites (CTRL and CFZ 3.2 nM, white arrows) and degenerated neurites (BTZ 2.8 nM, black arrows). Bar = 200 μm. (D) The graph represents the elongation of neurites untreated (CTRL), treated with BTZ 2.8 nM or CFZ 3.2 nM, after 48 h. Percentages are calculated on the respective lengths at 0 h **p < 0.01 versus CTRL; °°p < 0.01 versus CFZ 3.2 nM.
