Figure 9

NMR spectra. (A) ¹H NMR spectrum of 500 μM BTZ dissolved in 20 mM PB, MgCl₂, pH 7.4, d₆-DMSO 8% v/v, 37 °C; (B) STD NMR spectrum of 500 μM BTZ dissolved in 20 mM PB, MgCl₂, pH 7.4, d₆-DMSO 8% v/v, 37 °C; (C) STD NMR spectrum of 500 μM BTZ and tubulin α/β dimers 10 μM in polymeric form dissolved in 20 mM PB, MgCl₂, GTP 100 μM, pH 7.4, d₆-DMSO 8% v/v, 37 °C; (D) ¹H NMR spectrum of 500 μM CFZ dissolved in 20 mM PB, MgCl2, pH 7.4, d₆-DMSO 8% v/v, 37 °C; (E) STD NMR spectrum of 500 μM CFZ dissolved in 20 mM PB, MgCl₂, pH 7.4, d₆-DMSO 8% v/v, 37 °C; (F) STD NMR spectrum of 500 μM CFZ and tubulin α/β dimers 10 μM in polymeric form dissolved in 20 mM PB, MgCl₂, GTP 100 μM, pH 7.4, d₆-DMSO 8% v/v, 37 °C; (G) STDD NMR spectrum obtained by subtraction of spectrum E from spectrum F. All spectra were acquired at 600 MHz after at least 30 min sample incubation at 37 °C.