Figure 1
From: Increased hippocampal excitability in miR-324-null mice
Mir324 was knocked out in a C57/BL6 background in a 133 bp deletion. (a) The Mir324 locus and surrounding region in relation to the knockout. The positions from which the mature miR-324-5p and -3p arms are encoded are indicated and text in italics shows the locations of the protospacer-adjacent motifs used for the knockout. (b) Routine breeding and genotyping by PCR was used to maintain the stock, and allowed easy identification of whether each individual was heterozygous (HET), wild-type (WT) or miR-324-null (KO) with respect to Mir324. (c) The expression levels of Mir324 in hippocampus and neocortex tissue illustrate the lack of Mir324 in 13.5 month old male miR-324-null (KO) mice relative to WT mice. U6 was used as the control to normalise Mir324 levels between samples. Here, *** signifies p value ≤ 0.001, where significance was calculated using two-sided Student’s t-tests. (d) miR-324-5p is shown to be expressed in the murine hippocampal CA3 region by in situ hybridisation. Arrows indicate the cells in which miR-324-5p expression is detected as punctate red spots. Hippocampal slices stained for negative and positive controls (a scrambled probe and U6, respectively) are also shown.
