Figure 2

taken from 5 month old female miR-324-null and WT mice (N = 16 slices obtained from 6 mice per genotype). (a) Example 1 s traces of LFP recorded from a single WT and miR-324-null slice in the absence of KA (baseline) and following bath application of KA at 150 nM and 800 nM. In both genotypes, no spontaneous oscillations were seen before KA application, but clear gamma-frequency activity was evident at 150 nM. In the miR-324-null slices, “spikes” in the LFP are indicated by *. Oscillations subsequently collapsed at higher KA concentrations (800 nM KA). (b–d) Comparisons between genotypes of area power, peak amplitude and peak frequency showed no significant differences, although area power was close to the 5% significance threshold (p value = 0.068). KA concentration significantly affected all three metrics. Slices that did not show clear gamma-frequency oscillatory activity at a particular concentration were omitted from analysis at that concentration, and therefore for some KA concentrations N < 16. p Values were calculated using 2-way mixed ANOVAs, although for peak amplitude and area power, the data was first log₁₀ transformed as the initial data were not normally distributed.