Figure 3
From: Increased hippocampal excitability in miR-324-null mice
Increased excitability in hippocampal slices obtained from female 5 month old miR-324-null mice. (a) IIDs were observed in some slices from miR-324-null mice. (b) A far higher proportion of miR-324-null hippocampal slices showed IIDs or a high number of spikes than WT slices (N = 16 slices obtained from 6 mice per genotype). Any slice displaying at least one IID was classed as showing interictal activity, whilst slices showing no interictal activity but a high number of spikes were also grouped together. Slices were classed as showing high number of spikes if they had a greater number of spikes than the mean plus 2 SDs of spike number in the WT slices, for each KA concentration. For example, the mean plus 2 SDs of WT spikes at 150 nM KA was 15.0, and therefore any slices at 150 nM with more than 15 spikes were classed as having a high number of spikes. The most prominent distinction between genotypes is seen at 150 and 200 nM KA, at both of which 37.5% of miR-324-null slices displayed interictal activity, whereas the vast majority of WT slices at these concentrations showed no abnormal activity.
