Figure 2

(A–C) NDMS (non-metric multidimensional scaling) plots to see if results from the 13 laboratories cluster according to OTU assignment tool (A), OTU assignment database (B) and taxa assignment database (C). OTU assignment database was included for laboratories that used closed reference OTU picking. They generally compared their sequences against a reference database of sequences that clustered the reads into OTUs based on sequence similarity. Later, many laboratories assigned taxonomic identifiers to each of these OTUs using a separate database which had sequence data (sometimes not clustered into OTUs) and which taxa that sequence originated from.