Table 3 Primers used for this study.

From: An inter-laboratory study to investigate the impact of the bioinformatics component on microbiome analysis using mock communities

Strategy

Variable Regions

Primer

Sequence (5′–3′)

Positiona

β

1–2

Forward

GCTCAGATTGAACGCTGGCGG

22–358

GTTCAGATTGAACGCTGGCGG

 

GCTCAGGACGAACGCTGGCGG

 

GCTCAGGATTAACGCTGGCGG

 

GCTCAGGATGAACGCTGGCGG

 

GCTCAGAATGAACGCTGGCGG

 

GCTCAGGGTGAACGCTGGCGG

 

GCTCAGAGTGAACGCTGGCGG

 

Reverse

ACTGCTGCCTCCCGTAGGAGT

 

γ

4–6

Forward

GTGCCAGCAGYYGCGGTAATAC

518–1079

Reverse

CACRACACGAGCTGACGACA

 
  1. aBased on numbering from gene rrsH accession number AE014075 from E. coli CFT073 complete genome NCBI reference sequence: NC_004431.1
Back to article page