Figure 2

Adipocyte specific CaSR deficiency does not influence advanced stages of atherosclerosis. (a) Representation of the experimental workflow. Mice were crossed and offspring were injected with tamoxifen and fed a HFD for 12 weeks before analysis. (b) Schematic representation of the areas that were used for analysing atherosclerotic plaques. (c) Representative images and quantification of aortic root lesions in both AdipoqCre− Casr^flox Apoe^−/− and AdipoqCre+ Casr^flox Apoe^−/− mice (n = 9–10). Scale bar 500 µm. (d,e) Quantification of aortic arch lesions (n = 5) (d) and thoraco-abdominal aortic lesions (n = 9–10) (e) in AdipoqCre− Casr^flox Apoe^−/− and AdipoqCre+ Casr^flox Apoe^−/− mice. (f) Quantification of macrophage content of aortic root lesions in AdipoqCre− Casr^flox Apoe^−/− and AdipoqCre+ Casr^flox Apoe^−/− mice (n = 9). (g) Images to represent the presence of collagen in plaques in aortic roots and their quantification in AdipoqCre− Casr^flox Apoe^−/− and AdipoqCre+ Casr^flox Apoe^−/− mice (n = 10). Scale bar 250 µm. (h,i) Quantification of plasma triglycerides (h) and cholesterol (i) levels of AdipoqCre− Casr^flox Apoe^−/− and AdipoqCre+ Casr^flox Apoe^−/− mice (n = 10–11). Image processing was done using Image J 1.53 (https://imagej.nih.gov/ij/). Bar graphs are representation of mean ± SEM.