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Figure 1

From: Computational based design and tracking of synthetic variants of Porcine circovirus reveal relations between silent genomic information and viral fitness

Figure 1

(A) Flow diagram of the study: 1. Building computational models 2. Simulating/implementing the models on powerful servers 3. DNA pool synthesis 4. Generating live viral libraries. 5. Viral competition. 6. NGS and Read Count (RC) analysis. (B) The genome of PCV2 with ORF1—ORF4 marked. In gray—the edited region in the synthetic library (nucleotides 738 – 947 according to NCBI accession number KJ128273). This region includes the Ori (origin of replication, marked in a black region) and the beginning of ORF1. The Ori region includes the stem-loop structure, marked in blue. The splicing sites of rep', rep3 and cap are marked in black lines. (C) A heatmap diagram of the DNA sequences in the oligonucleotide library (nucleotides 698 – 947 according to NCBI accession number KJ128273) of the five hundred variants in the synthetic library. This region includes the edited region with an inception of 40 nucleotides identical to the wildtype (see Methods section); the heatmap was generated by Matlab.

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