Figure 4 | Scientific Reports

Figure 4

From: The miR-378c-Samd1 circuit promotes phenotypic modulation of vascular smooth muscle cells and foam cells formation in atherosclerosis lesions

Figure 4

miR-378 controls VSMCs phenotypic modulation thought Samd1. (A) Western blot analysis of Samd1 expression in VSMCs stimulated with PDGF-BB. β-actin serves as a loading control. (B) Western blot analysis of Samd1 expression in VSMCs stimulated with serum-starvation (0.1%). β-actin serves as a loading control. (C) VSMCs stimulated with PDGF-BB were further transfected with miR-378c mimic, followed by western blot analysis of Samd1 expression. β-actin serves as a loading control. (D) VSMCs stimulated with PDGF-BB were further transfected with Samd1 shRNA or NTC, VSMCs differentiation markers were analyzed by qRT-PCR. Data were presented as mean ± SD, n = 4. *P < 0.05 as compared with non-PDGF treated group; #P < 0.05 as compared with PDGF-treated NTC group. (E) PDGF-BB stimulated VSMCs were further transfected with Samd1 shRNA or NTC, cell numbers were determined. Data were presented as mean ± SD, n = 4. *P < 0.05 as compared with PDGF-treated NTC group. (F) PDGF-BB stimulated VSMCs treated with miR-378c inhibitor were further transfected with Samd1 shRNA or NTC, followed by qRT-PCR analysis of VSMC differentiation markers. Data were presented as mean ± SD, n = 4. *P < 0.05 as compared between the indicated group. (G) PDGF-BB stimulated VSMCs treated with miR-378c inhibitor were further transfected with Samd1 shRNA or NTC, cell numbers were determined. Data were presented as mean ± SD, n = 4. *P < 0.05 as compared between the indicated group.

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