Figure 5
From: AAV-mediated YAP expression in cardiac fibroblasts promotes inflammation and increases fibrosis
YAP promotes inflammatory-associated gene expression in cardiac fibroblasts. (a–f) Adenoviral-mediated expression of YAP in NRCFs increased select cytokine and chemokine mRNA expression. (g) ChIP analysis demonstrates YAP enrichment at the Ccl2 gene promoter in NRCFs. (h) A Ccl2-luciferase reporter construct was used to detect promoter activation. Increased YAP expression significantly increased luciferase activity in HEK293 cells, which was significantly reduced in the presence of verteporfin (1 μM). N = 3 experimental replicates. *p < 0.05. ns not significant.
