Figure 4
From: Prematurity alters the progenitor cell program of the upper respiratory tract of neonates
Diminished mitochondrial activity in preterm NP-derived basal cells. (a) Seahorse analysis of cellular oxygen consumption rate (OCR) over time in cultured NP-derived basal cells from preterm and term subjects; effects of selected compounds on different steps of the mitochondrial respiratory chain. OCR quantification at Baseline, Oligomycin-treatment, FCCP treatment and the cellular respiratory control ratio calculated as a ratio of the FCCP rate to Oligomycin rate. Each point represents an individual infant (n = 4 for term infants and n = 7 for premature infants). Open circle marks the premature infant with the youngest gestational age in the study (25 weeks gestational age). Bar graphs are mean ± SEM; p-values of the Mann–Whitney U test are shown. (b) MitoTracker Green (MTG) FM fluorescence staining in preterm and term confluent basal cells in culture (DAPI, blue). Graphs: bars are mean (± SEM) of MTG per nucleus in preterm and term cells; p-value (depicted) Student t test.
