Figure 2 | Scientific Reports

Figure 2

From: Withaferin A inhibits lymphocyte proliferation, dendritic cell maturation in vitro and prolongs islet allograft survival

Figure 2

Effects of WA treatment on prevention of stimulated mouse lymphocyte proliferation. CD3+ T cell proliferation detected by flow cytometry analysis of CFSE-stained cells after 5 days of culture for (A) T cells from splenocytes isolated from BALB/c mice and stimulated by CD3/CD28 beads with or without WA 0.5 μg/mL treatment; (B) lymph node cells isolated from BALB/c mice and stimulated by CD3/CD28 beads with or without WA 0.5 μg/mL treatment; and (C) T cells from splenocytes isolated from C57BL/6N mice and stimulated by CD3/CD28 beads with or without WA 0.5 μg/mL treatment. Representative histograms from three independent experiments are shown. (D) Proliferation rate of BALB/c T cells, BALB/c lymphnode cells and C57BL/6N T cells are shown. **P < 0.01.

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