Figure 5
Inhibitory effect of WA treatment against human DC maturation. Human PBMCs isolated from healthy donors were cultured in a medium containing 0.15 or 0.25 μg/mL WA for the generation of mature DCs. Expression of (A) CD83, (B) CD86, and (C) HLA-DR was detected by flow cytometry after 48 h of culture. The dotted and clear histograms represent control cells and WA 0.15 μg/mL treated cells, respectively. The gray area represents WA 0.25 μg/mL–treated cells. (D–F) Median fluorescent intensity (MFI) of (D) CD83, (E) CD86, and (F) HLA-DR. Control (circle), WA0.15 (square), WA0.25 (gray triangle). *P < 0.05, **P < 0.01, n.s.; not significant. n = 3 independent experiments.
