Figure 7
Protective effect of WA-pretreated human stress islet exosomes in the activation of human macrophages. Approximately 1 µg exosomes isolated from normal islets after 24 h of culture (EXON Islets), exosomes isolated from cytokine cocktail (CC)-induced stressed islets (EXOS Islet), and exosomes isolated from WA 1.0 μg/mL-pretreated and CC-induced stressed islets (EXOWA+S Islet) were used for activation of human macrophages (THP cells), cultured for 6 h. The relative intracellular mRNA expression of iNOS and COX-2 was quantified by quantitative polymerase chain reaction. The data represent mean ± standard error of the mean. ***P < 0.001 compared to EXON Islets, ###P < 0.001 compared with EXOS Islets.
