Figure 4
Changes in the number of inhibitory synapses in Scn1a+/− neurons. (a) Representative traces of responses to 0.5 M hypertonic sucrose solution (10 s, black bar) in an autapse neuron of either wild-type (WT) or Scn1a+/− mice. (b) The size of the readily releasable pool (RRP) of autaptic neurons of either WT or Scn1a+/− mice (WT: 1.91 ± 0.12 nC, n = 91; Scn1a+/−: 1.92 ± 0.12 nC, n = 92/N = 5 cultures). (c) Vesicular release probability (Pvr) in single autaptic neurons of either WT or Scn1a+/− mice (WT: 29.99 ± 2.31%, n = 91; Scn1a+/−: 24.01 ± 1. 66%, n = 92/N = 5 cultures, *p < 0.05). (d) Representative images of autaptic neurons immunostained for the inhibitory nerve terminal marker vesicular GABA transporter (VGAT; red) and the dendritic marker microtubule-associated protein 2 (MAP2; green). Parts of the top row (scale bar 50 μm) are enlarged in the bottom row (scale bar 20 μm). (e) The number of VGAT–positive synaptic puncta in autaptic neurons of either WT or Scn1a+/− mice (WT: 152.23 ± 7.91, n = 70, Scn1a+/−: 117 ± 10.61, n = 74/N = 8 cultures, *p < 0.05).
