Figure 1 | Scientific Reports

Figure 1

From: Modular barcode beads for microfluidic single cell genomics

Figure 1

Barcode bead fabrication overview. (a) Barcode beads form the backbone of microfluidic single cell genomic protocols. Close packing enables efficient bead loading. (b) Organization of the barcode sequence on the beads. The elements are modular and can be removed or replaced with other sequence blocks if desired. The 0–3 bp spacer introduces a barcode specific frame shift that helps with cluster detection in Illumina’s sequence technolgoy. (c) DNA ligation with T4-ligase is used to minimize the footprint of the split-pool barcode fragments enabling more split pool cycles per length.

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