Table 1 Bead fabrication cost overview: bead costs of InDrops beads are compared to the here described approach.
From: Modular barcode beads for microfluidic single cell genomics
InDrops gel beads | 746496 | Barcode space | |||||
|---|---|---|---|---|---|---|---|
Scale order | Yield guarantee | Set-up cost | Bead volume yield | Cost per ml bead | Cost per experiment (~ 20,000 cells) | ||
1 | InDrops Acrydite p | 10,000 | 125 nmol | 3218.8 $ | 5.7 ml | 569.7 $/ml | 28.48 $ |
18 | Barcode Plates | 10 | – nmol | 15,042.6 $ | 80.0 ml | 188.0 $/ml | 9.40 $ |
1 | BST 2.0 | 8000 | – units | 283.0 $ | 8.6 ml | 33.0 $/ml | 1.65 $ |
1 | dNTP | 40 | – umol | 250.0 $ | 14.8 ml | 16.9 $/ml | 0.85 $ |
Total | 18,794.4 $ | 807.7 $/ml | 40.38 $ | ||||
Ligation gel beads | 884736 | Barcode space | |||||
|---|---|---|---|---|---|---|---|
Scale order | Yield guarantee | Set-up cost | Bead volume yield | Cost per ml bead | Cost per experiment (~ 20 000 cells) | ||
1 | pBB1 | 10,000 | 1600 nmol | 1738.4 $ | 72.3 ml | 24.0 $/ml | 1.20 $ |
3 | Barcode Plates | 25 | – nmol | 902.9 $ | 12.0 ml | 75.2 $/ml | 3.76 $ |
3 | Splint Plates | 100 | – nmol | 1199.5 $ | 50.0 ml | 24.0 $/ml | 1.20 $ |
1 | pBB2 (phos-lig_start) | 10,000 | 1600 nmol | 1107.2 $ | 72.3 ml | 15.3 $/ml | 0.77 $ |
1 | pBB3 (release-3C) | 1000 | 100 nmol | 102.0 $ | 16.0 ml | 6.4 $/ml | 0.32 $ |
1 | pBB4 end splint | 1000 | 200 nmol | 21.0 $ | 10.7 ml | 2.0 $/ml | 0.10 $ |
1 | pBB5 (phos-RNA) | 1000 | 200 nmol | 126.9 $ | 10.7 ml | 11.9 $/ml | 0.59 $ |
1 | T4 PNK | 2500 | – units | 228.0 $ | 13.0 ml | 17.5 $/ml | 0.88 $ |
1 | T4 Ligase | 100,000 | – units | 256.0 $ | 4.2 ml | 61.4 $/ml | 3.07 $ |
1 | USER | 250 | – units | 297.0 $ | 8.3 ml | 35.6 $/ml | 1.78 $ |
Total | 5978.9 $ | 273.4 $/ml | 13.67 $ | ||||
