Figure 7
From: CD13 is a critical regulator of cell–cell fusion in osteoclastogenesis
Immunoblot analysis of sustained expression of fusion-regulatory proteins in CD13-deficient OC over time. Expression of the fusion-promoting proteins dynamin, DC-STAMP (DCST1) and CD9 is aberrantly sustained in CD13KO but not in WT multinucleated osteoclasts (a,b) in presence of M-CSF and RANKL. A cropped image is presented, see Supplementary Fig. S7 for full-length blots and Supplementary Fig. S8 for cropped replicates. (c,d). CD13 expression is unaltered in BM-derived OCs in response to M-CSF and RANKL stimulation over time. Blots were imaged by ChemiDoc Imaging system version 3.0.1 (https://www.bio-rad.com/en-us/category/chemidoc-imaging-systems?ID=NINJ0Z15) (Biorad). A cropped image is presented, see Supplementary Fig. S9 for full-length blots and cropped replicates. Data represents average of two isolates. N = 3/genotype. **p < 0.01, *p < 0.05.
