Table 2 Conditions of P. donghuensis P482 culture used in reference gene selection study.
No. | Medium | Culturing time, temperature, and growth phase |
|---|---|---|
1. | M9 + 0.4% glucose | 12 h, 28 °C, late log phase |
2. | M9 + 0.4% glucose + 30 µM FeSO4 | 12 h, 28 °C, late log phase |
3. | M9 + 0.4% glucose + 30 µM FeCl3 | 12 h, 28 °C, late log phase |
4. | M9 + 0.4% glycerol | 30 h, 28 °C, late log phase |
5. | M9 + 0.4% glycerol + 30 µM FeSO4 | 22 h, 28 °C, late log phase |
6. | M9 + 0.4% glycerol + 30 µM FeCl3 | 30 h, 28 °C, late log phase |
7. | M9 + 0.4% glucose + maize root exudates | 12 h, 28 °C, late log phase |
8. | M9 + 0.4% glucose + tomato root exudates | 12 h, 28 °C, late log phase |
9. | 10% TSB | 10 h, 28 °C, mid-log phase |
10. | 10% TSB | 15 h, 28 °C, stationary phase |
11. | 10% TSB | 16 h, 22 °C, late log phase |
12. | LB | 16 h, 28 °C, late log phase |
