Table 4 Bacterial strains and plasmids used in the study.
Strain | Origin/features | References |
|---|---|---|
Pseudomonas strains | ||
Pseudomonas donghuensis P482 | Tomato plant rhizosphere (Poland), wild type (wt) | Krzyżanowska et al.9 |
Pseudomonas vranovensis DSM16006T | Soil (Czech Republic); no antimicrobial activity (negative control strain) | Tvrzová et al.107 |
Plant pathogenic strains | ||
Dickeya solani IFB0102 | Potato plant (Poland) | Sławiak et al.108 |
Pseudomonas syringae pv. syringae Pss762 | Apricot (Poland) | Kałużna et al.109 |
Strains used in genetic engineering | ||
Escherichia coli ST18 | Donor strain for a biparental mating: pro thi hsdR + TprR SmrR; chromosome:RP4-2 Tc::Mu-Kan::Tn7/λpir ΔhemA | Thoma et al.110 |
Genetically modified strains | ||
KN3318 | P. donghuensis P482 with insertion of pKNOCK backbone in gacA gene locus | Krzyżanowska et al.10 |
KN4705, KN4706, KN4709 | P. donghuensis P482 with insertion of pKNOCK backbone in respective loci (genes involved in a potential 7-HT production) | Krzyżanowska et al.10 |
KN1009, KN3755 | P. donghuensis P482 with insertion of pKNOCK backbone in respective loci (genes involved in pyoverdine production) | Krzyżanowska et al.10 |
KN4240, KN4243 | P. donghuensis P482 with insertion of pKNOCK backbone in respective loci (“cluster 17” genes) | This study |
Plasmids | ||
pKNOCK-Km | 2098 bp KmR suicide vector for site directed mutagenesis | Alexeyev111 |
pKN4240 | 2492 bp KmR pKNOCK-Km vector with 394 bp fragment of BV82_4240 gene (primers F_XbaI_KN4240 / R_XhoI_KN4240) in the XbaI_XhoI cloning site | This study |
pKN4243 | 2490 bp KmR pKNOCK-Km vector with 392 bp fragment of BV82_4243 gene (primers F_XbaI_KN4243 / R_XhoI_KN4243) in the XbaI_XhoI cloning site | This study |
