Figure 3

Splenectomy increased inflammatory markers in mesenteric PVAT (mPVAT) of mice fed a high-fat diet (HFD). (a, d, f) Representative dot plots of flow cytometry and populations of (b) CD4⁺ and (c) CD8⁺ T lymphocytes (percentage of CD3⁺ cells), (e) B220⁺ B lymphocytes (percentage of CD45⁺ cells) and (g) CD206^-CD11c⁺ (M1) and (h) CD206⁺CD11c⁻ (M2) macrophages (percentage of F4/80⁺ cells) in the mPVAT of sham-operated (SHAM) or splenectomized (SPX) mice fed with standard diet (SD) or HFD. Results from two different experiments (n = 5 to 9 per group). (i–m) Cytokine levels in mPVAT of SD-SHAM, SD-SPX, HFD-SHAM and HFD-SPX mice. (n = 5 to 7 per group). Data were expressed as mean ± SEM. ANOVA: *P < 0.05, **P < 0.01 and ***P < 0.001, SD-SPX and HFD-SHAM versus SD-SHAM; ^$P < 0.05, ^$$P < 0.01, ^$$$P < 0.001 and ^$$$$P < 0.0001, HFD-SPX versus SD-SPX; ^#P < 0.05 and ^##P < 0.01, HFD-SPX versus HFD-SHAM.