Figure 6
Quantification of gapmer uptake in HEK 293 T cells after 24 h continuous exposure, evaluated using flow cytometry. All data were collected using the same excitation power (excitation at 405 nm) and detector settings. To enable comparisons of the FBA-labeled gapmer concentrations inside cells, a relative uptake was calculated based on the observed mean fluorescence intensity (MFI, see Supplementary Fig. S24 for the untreated data). Briefly, this was achieved by (1) subtracting the corresponding MFI from the negative control (unmodified gapmer U at equal concentration), (2) dividing by the brightness of the single-stranded gapmer (B) and (3) dividing by a spectral correction factor (SCF), see supplementary Sect. 3.5 for details. (a) Dose-dependent relative uptake. (b) Relative uptake at the top concentration in (a). Error bars are propagated standard deviations (N = 6) based on the cytometry- and brightness data (Supplementary Fig. S18). *Probability for equal mean from a paired sample t-test.
