Table 2 Comparison of NfL concentrations at different freezing intervals.

From: Seven day pre-analytical stability of serum and plasma neurofilament light chain

 

[sNfL] freezing interval: immediate

[sNfL] freezing interval: 3 days RT

[sNfL] freezing interval: 3 days 4–8 °C

[sNfL] freezing interval: 7 days RT

p-valuea

Mean (pg/mL)

95% CI

Mean (pg/mL)

95% CI

Mean (pg/mL)

95% CI

Mean (pg/mL)

95% CI

(a)

Whole cohort

14.6

9.7–19.5

15.5

9.8–21.2

16.0

10.3–21.8

15.5

10.1–20.9

0.192

Lowest quartile

5.2

4.8–5.6

5.3

4.4–6.1

5.4

4.9–5.4

5.6

4.9–6.4

0.633

Highest quartile

34.2

22.6–45.7

36.8

20.8–52.7

38.5

23.7–53.3

36.8

23.5–50.1

0.422

 

[pNfL] freezing interval: immediate

[pNfL] freezing interval: 3 days RT

[pNfL] freezing interval: 3 days 4–8 °C

[pNfL] freezing interval: 7 days RT

p-valuea

Mean (pg/mL)

95% CI

Mean (pg/mL)

95% CI

Mean (pg/mL)

95% CI

Mean (pg/mL)

95% CI

(b)

Whole cohort

12.0

8.1–15.9

12.5

8.3–16.8

12.8

8.1–17.5

12.5

8.4–16.6

0.385

Lowest quartile

4.8

4.1–5.5

4.8

4.4–5.3

4.3

3.7–4.8

5.0

4.4–5.5

0.071

Highest quartile

28.8

18.5–39.1

30.3

19.6–41.1

31.6

18.1–45.2

30.0

19.5–40.5

0.541

  1. Mean values and 95% confidence intervals (95% CI) for (a) serum neurofilament light chain (sNfL) and (b) plasma neurofilament light chain (pNfL).
  2. CI confidence interval, pNfL plasma neurofilament light chain concentration (pg/mL), RT room temperature, sNfL serum neurofilament light chain concentration (pg/mL).
  3. aCalculated by repeated measurement ANOVA.
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