Figure 2 | Scientific Reports

Figure 2

From: Functional characterization of a loss-of-function mutant I324M of arginine vasopressin receptor 2 in X-linked nephrogenic diabetes insipidus

Figure 2

The V2R proteins carrying I324M mutant lost the ability expressed on the plasma membrane and almost entirely retained in the ER. COS7 cells were transiently expressed Myc-tagged-WT-V2R (WT) or Myc-tagged-I324M-V2R (I324M), respectively. (A) The fluorescence imaging of ER location of WT and I324M co-stained with PDI respectively using anti-Myc rabbit polyclonal antibody and anti–PDI mouse monoclonal antibody. (B) The fluorescence imaging of Golgi location of WT and I324M co-stained with GM130 respectively using anti-Myc rabbit polyclonal antibody and anti–GM130 mouse monoclonal antibody. V2R was shown in green; PDI and GM130 were shown in red; The superposition of V2R and PDI or GM130 was shown in orange. PDI: protein disulfide isomerase, a marker of endoplasmic reticulum (ER); GM130: a marker of Golgi apparatus; DAPI: a marker of nucleus. Representative of 3 separate experiments.

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