Figure 6 | Scientific Reports

Figure 6

From: Functional characterization of a loss-of-function mutant I324M of arginine vasopressin receptor 2 in X-linked nephrogenic diabetes insipidus

Figure 6

The impaired maturity and cAMP signaling activity of I324M mutant were partially restored by SR49059 and alvespimycin. HEK293T cells were transiently transfected with plasmid encoding pcDNA3.1 (Ctr), WT-V2R (WT) or I324M-V2R (I324M). 24 h after transfection, the cells were incubated with or without SR49059 (1 μM) for 16 h and alvespimycin (1 μM) for 20 h before treated with or without DDAVP (100 nM). (A) Results were shown as bar graph and data represent the mean ± SEM using Two-way ANOVA were obtained from three independent experiments. ****P < 0.0001, **P < 0.01. (B) V2R expression of WT and I324M groups in the stimulation with or without SR49059 (1 μM) and alvespimycin (1 μM). Representative of 3 separate experiments.

Back to article page