Figure 6 | Scientific Reports

Figure 6

From: C1q/TNF-related protein-9 ameliorates hypoxia-induced pulmonary hypertension by regulating secretion of endothelin-1 and nitric oxide mediated by AMPK in rats

Figure 6

Knockdown of AMPK expression inhibited CTRP9-induced alterations in expression of p-AMPK, p-eNOS, p-ERK1/2, NO and ET-1. (A–C) Western blotting of expression of AMPK and p-AMPK (A, n = 3; blots were cropped from different parts of the same gel), eNOS and p-eNOS (B, n = 3; blots were cropped from different gels), ERK1/2 and p-ERK1/2 (C, n = 3; blots were cropped from different gels) in rat PMVECs. The protein ratio of p-AMPK to AMPK, p-eNOS to eNOS, and p-ERK1/2 to ERK1/2 was calculated. (D, E) Levels of NO (n = 3) and ET-1 (n = 4) in PMVECs culture medium were examined. Data are the mean ± SEM. ※P < 0.05 vs. H + LV-Control group; #P < 0.05 vs. H + LV-Control + CTRP9 group. H hypoxia, LV-Control lentivirus control, LV-shAMPK lentivirus with AMPK interruption.

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