Figure 3 | Scientific Reports

Figure 3

From: Interactions between the flavescence dorée phytoplasma and its insect vector indicate lectin-type adhesion mediated by the adhesin VmpA

Figure 3

Observation by laser scanning microscopy of fluorescent lectins binding salivary gland carbohydrates of FDP-infected E. variegatus. Cell nuclei were stained with DAPI (blue), actin filaments with Alexa 568-phalloidin (red), phytoplasmas with anti-VmpA antibodies and Alexa 633-secondary antibodies (cyan), and FITC-lectins are colored green. (a) Overlay pictures of infected salivary gland cells stained with the lectin indicated in each picture. Arrows indicate the FDP-infected cells, and arrowheads indicate the cells recognized by the lectin used. (b) View of the infected salivary gland surfaces stained with the lectin indicated in each picture. Scale bar 100 µm.

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