Figure 4 | Scientific Reports

Figure 4

From: Interactions between the flavescence dorée phytoplasma and its insect vector indicate lectin-type adhesion mediated by the adhesin VmpA

Figure 4

Magnification of salivary glands of FDP-infected E. variegatus stained with fluorescent LEL, GNA and LCA lectins by laser scanning microscopy with an Airyscan detector. FDPs were stained with anti-VmpA antibodies and Alexa 633-secondary antibodies and (cyan), cell nuclei with DAPI (blue), actin filaments with Alexa 568-phalloidin (red) and glycoconjugates with FITC-lectins LEL, GNA and LCA (green). (a) View of the infected salivary gland cell surface. (b) View of the inside of infected salivary gland cells. Arrows indicate structures resembling apical membranes described by Koinuma et al.16 around which phytoplasmas are grouped. Arrowheads indicate phytoplasmas that crossed the apical plasma membrane. Scale bar 10 µm.

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