Figure 6 | Scientific Reports

Figure 6

From: Interactions between the flavescence dorée phytoplasma and its insect vector indicate lectin-type adhesion mediated by the adhesin VmpA

Figure 6

Interaction of VmpA with Euva-6 cells in the presence of sugar and lectins. (a) Adhesion of VmpA-His6 proteins to Euva-6 cells measured by ELISA in the presence of sugars. The recombinant proteins were first incubated with different concentrations of sugars indicated below the graphs before being added to Euva-6 cells. Boxplots with different letters are significantly different under the ANOVA test of the R commander package of R software version 4.0.3 (R: A language and Environment for statistical computing, R Core Team, R Foundation for Statistical Computing, Vienna, Austria, 2020, https://www.R-project.org). The normal distribution of the value was previously verified using the Shapiro–Wilk normality test of R software (R commander package). (b) Adhesion of VmpA-His6-coated beads to Euva-6 cells in competition with lectins. Euva-6 cells were preincubated with the lectins indicated above the graphs before being incubated with fluorescent VmpA-His6-coated beads. 0 indicates that no lectin was incubated with insect cells. The P values according to the Kruskal–Wallis rank sum test of the software R (R commander package) are indicated above the boxplots.

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