Figure 6
Effect of 12-week binge sucrose intake on astroglial cells in rats. Immunohistological analysis revealed no effect of sucrose diet on the astrocyte coverage in the (A) motor cortex, (B) amygdala, (D) hippocampus and (E) thalamus as stained with GFAP (red). (A’–B’ and D’–E’) shows representative staining in each of these regions in the normal diet (left panel) and sucrose diet (right panel) groups, scale bar = 100 µm. (C) The area covered and (F) fluorescence intensity of astrocytes staining was reduced in the hypothalamus of the sucrose diet rats. (C’) Representative staining of the astroglial marker GFAP in the hypothalamus in the normal diet (left panel) and sucrose diet (right panel) groups, scale bar = 100 µm. All values are presented as mean area covered with GFAP positive staining ± SEM or mean GFAP fluorescence intensity ± SEM in the brain region imaged analysed with Student’s two-tailed t-test. *p < 0.05, **p < 0.01 compared to controls. All values presented are from n = 9 per group.
