Figure 1 | Scientific Reports

Figure 1

From: Novel electronic biosensor for automated inoculum preparation to accelerate antimicrobial susceptibility testing

Figure 1

Accelerated AST workflow with eQUANT. The current laboratory workflow starts with a positive blood culture (PBC, red box) followed by a Gram stain (GS, light grey box) and rapid organism identification if available (rID, dark grey box). Bacteria from the PBC are subcultured and a 0.5 McF is prepared from colonies. The 0.5 McF is then used to lawn an agar plate for disk diffusion or utilized in an automated AST platform. On average, AST results are available in 10–18 h. With the eQUANT method, the need for the subculture step is eliminated, and the eQUANT inocula is directly generated from the PBC as soon as the organism is identified.

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