Figure 1 | Scientific Reports

Figure 1

From: Heparan sulfate proteoglycan glypican-1 and PECAM-1 cooperate in shear-induced endothelial nitric oxide production

Figure 1

AFM pulling on glypican-1, but not PECAM-1, induced NO production in HUVEC. HUVEC monolayers were probed with rabbit anti-glypican-1, rabbit anti-PECAM-1, or normal rabbit IgG tipless AFM cantilevers. (A) Mean % activation ± SEM are shown (n = 27 for all groups). *P < 0.05 versus static conditions (0% activation) using two-tailed one-sample t-test. The dotted line is mean % activation of NO ± SEM for cells stimulated with fluid shear stress for 30 min (n = 16). (B) Representative confocal max intensity Z stack projections are shown for HUVEC with DAF 2T (green) fluorescence counterstained with DAPI (blue). The top row shows cells probed with functionalized AFM tips, while the bottom row shows unprobed cells from the same slide, approximately 2 mm away from probing location.

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