Figure 4
AFM pulling with glypican-1 antibody on PECAM-1 deficient HUVEC does not induce NO production. HUVEC monolayers were probed using AFM pulling with rabbit anti-glypican-1 tipless cantilevers. Mean % activation of NO production and SEM are shown for negative control siRNA transfected cells (n = 27) and PECAM1 siRNA transfected cells (n = 27). The dotted line marks the mean % activation of NO ± SEM for cells stimulated with fluid shear stress for 30 min (n = 16). *P < 0.05 versus static conditions (0% activation) using two-tailed one-sample t-test for bars and two-tailed student’s t-test for shear line.
