Table 2 Primer pairs’ sequences of mice genes used in RT-qPCR.
Proteins | Genes | Forward primer sequence | Reverse primer sequence |
|---|---|---|---|
GAPDH | Gapdh | TGTGTCCGTCGTGGATCTGA | TTGCTGTTGAAGTCGCAGGAG |
TG2 | Tgm2 | ATCCTGGACCCACTTCTTCTT | CTCTAGGCTGAGACGGTACAG |
IFN-γ | Ifng | ATGAACGCTACACACTGCATC | ATGAACGCTACACACTGCATC |
IL-4 | Il4 | GGTCTCAACCCCCAGCTAGT | GCCGATGATCTCTCTCAAGTGAT |
IL-5 | Il5 | GCCGATGATCTCTCTCAAGTGAT | CGGACAGTTTGATTCTTCAGTATG |
IL-17A | Il17a | GGACTCTCCACCGCAATGAA | TTTCCCTCCGCATTGACACA |
IL-9 | Il9 | CTTGTGTCTCTCCGTCCCAAC | ACTATCCTTTTCACCCGATGGA |
IL-1α | Il1a | ACGTCAAGCAACGGGAAGAT | AAGGTGCTGATCTGGGTTGG |
TNF-α | Tnfa | CAGGAGGGAGAACAGAAACTCCA | CCTGGTTGGCTGCTTGCTT |
IL-6 | Il6 | TGGAGTCACAGAAGGAGTGGCTAA | TCTGACCACAGTGAGGAATGTCCAC |
IL-8 | Il8 | TGGGTGAAGGCTACTGTTGG | AGCTTCATTGCCGGTGGAAA |
IL-10 | Il10 | GCTGGACAACATACTGCTAACC | ATTTCCGATAAGGCTTGGCAA |
COX-2 | COX2 | TTCCAATCCATGTCAAAACCGT | AGTCCGGGTACAGTCACACTT |
mPGES-1 | Ptges | ATTTCCGATAAGGCTTGGCAA | TCACTCCTGTAATACTGGAGGC |
Eotaxin | Eotaxin | GAATCACCAACAACAGATGCAC | ATCCTGGACCCACTTCTTCTT |
MCPT4 | Mcpt4 | TAGACCACATTCTCGCCCTTA | GGATTCTGTCTTGCTCACATCA |
MCPT5 | Mcpt5 | GAGATCATTGGAGGCACGGAG | ACTGTTATAGACCTTCCCGCAC |
MCPT6 | Mcpt6 | CTGGCTAGTCTGGTGTACTCG | CCAGGGCCACTTACTCTCA |
MCPT7 | Mcpt7 | GCCAATGACACCTACTGGATG | GAGCTGTACTCTGACCTTGTTG |
CCL2 | Ccl2 | TTAAAAACCTGGATCGGAACCAA | GCATTAGCTTCAGATTTACGGGT |
CCL5 | Ccl5 | GCTGCTTTGCCTACCTCTCC | TCGAGTGACAAACACGACTGC |
VACM-1 | Vacm1 | GCCCACTAAACGCGAAGGT | ACTGGGTAAATGTCTGGAGCC |
SCF | SCF | AGCTTGACTACTCTTCTGGACA | TGGCCTCTTCGGAGATTCTTTT |
c-KIT | c-KIT | GCCTGACGTGCATTGATCC | AGTGGCCTCGGCTTTTTCC |
P2X2 | P2rx2 | ACAGAACTGGCACACAAGGG | CAGGGTCATACTTGGGGTCG |
P2X3 | P2rx3 | CAGGGTCATACTTGGGGTCG | CGAGCTGATGATGGTGGGAA |
TRPA1 | Trpa1 | CGGATGCACACCTCTCCATT | TGCAGGGGCGACTTCTTATC |
TRPV1 | Trpv1 | CCGGCTTTTTGGGAAGGGT | GAGACAGGTAGGTCCATCCAC |
TRPV4 | Trpv4 | GGACCCTGGCAAGAGTGAAA | AAGCCTGGACTATCTGTGCG |
