Table 4 (a) Validation results of PCR models for multiple age-linked peaks of three body fluids, (b) validation results of PLSR models for multiple age-linked peaks of three body fluids.

Fluids	Cross-validation		External Prediction		RPD
Fluids	R²	RMSECV (min)	R²	RMSEP (min)	RPD
(a) Principal component regression (PCR) analysis table
Semen (100%)	0.9935	0.2346	0.9939	0.2201	13.50
Semen (40%)	0.9390	0.7167	0.9415	0.6840	20.53
Saliva (100%)	0.9885	0.3742	0.9891	0.3568	9.64
Saliva (40%)	0.9973	0.1517	0.9973	0.1458	19.53
Urine (100%)	0.9948	0.2084	0.9951	0.1983	15.01
Urine (40%)	0.9518	0.7659	0.9532	0.7383	4.73
(b) Partial least square regression (PLSR) analysis table
Semen (100%)	0.9935	0.2333	0.9940	0.2189	13.57
Semen (40%)	0.9957	0.1897	0.9970	0.1546	19.26
Saliva (100%)	0.9889	0.3737	0.9891	0.3561	9.67
Saliva (40%)	0.9969	0.1618	0.9973	0.1457	20.44
Urine (100%)	0.9950	0.2055	0.9952	0.1953	15.24
Urine (40%)	0.9523	0.7622	0.9540	0.7327	4.78

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