Figure 12
From: Identification and function of ETH receptor networks in the silkworm Bombyx mori
Temporal changes and spatial distribution of ETHRs expression. (a) Relative transcripts levels of ETHR-A and ETHR-B in the CNS measured at different time points in pharate or ecdysed 5th instar larvae. 0 h represents ecdysis onset. RNA used for RT-qPCR was extracted from CNS with attached CA and H-organ. (b) Expression levels of ETHR-A and ETHR-B in different tissues of pharate 5th instar larvae. Error bars indicate standard error of the mean (n = 3). CNS (central nervous system), GUTant (anterior gut), GUTpost (posterior gut), GonF (female gonads) GonM (male gonads), FB (fat body), Epid (epidermis), MT (Malpighian tubules). (c–e) ETHR-A and ETHR-B expression in MT cells attached to surface of the Malpighian tubules. Scale bars c, e = 50 µm, d = 25 µm.
