Figure 4
From: Identification and function of ETH receptor networks in the silkworm Bombyx mori
ETHR-A expression in the CNS of pharate pupae. (a–g’) Neurons detected by ISH with ETHR-A probe were identified by subsequent staining with various antibodies. (a, a′) ETHR-A transcript detected in EH-producing VM neurons (arrows; green) and in two clusters of 20–30 small neurons in the brain (arrowheads). (b, b′) ETHR-A expression in two pairs of NS-27 producing bursicon and CCAP (arrows; yellow) or bursicon only (arrows; red), posterior IN-704 containing CCAP (small arrows; green) and in ∼20 unidentified neurons of the SG. (c, c’) Colocalization of ETHR-A with bursicon and CCAP in NS-27 (large arrows; yellow) and bursicon only in IN-704 of the TG2 (small arrows; red). (d, d’) Unidentified pair of ETHR-A neurons in the AG1 failed to react with antibodies to DH (green) and MIP (red). (e–g´) ETHR-A expression in IN-704 stained with antibody to MIPs (arrows; red) in the AG2-7. Note the absence of ETHR-A in cells L2,3 producing kinins, DHs and MIPs (small arrowheads; green/yellow). (g, g’) Colocalization of ETHR-A and MIP in VL8 cells (arrowheads; red) and PM9 (large arrows; red) in the posterior TAG. ETHR-A was detected in additional 4–5 pairs of unidentified neurons. Scale bars a, a´ = 100 µm, b–g´ = 50 µm.
