Figure 1
The design and activation concept of KIPPIS for detecting peptide aptamer–polypeptide interaction. (a) The constructs of chimeras with a helper module (FKBPF36V), a protein of interest [POI: peptide aptamer (A) or polypeptide (B)], and the c-kit intracellular domain (c-kit ICD). The helper ligand supports the formation of stable complexes. Flexible linkers (G4S)5 and (G4S)2 were employed to link the helper module/POI and POI/c-kit ICD, respectively. (b) The architectures of retroviral vectors. LTR long terminal repeat. Psi (Ψ); packaging signal. V5/Myc and HA/FLAG; tag peptides. IRES internal ribosome entry site. PuroR/NeoR; drug resistance gene against puromycin/neomycin.
