Figure 3
Detection of the endogenous/synthetic/viral peptide aptamer–polypeptide interactions based on cell proliferation. The chimeras expressed in the Ba/F3 transductants are shown as the abbreviated names. A cell proliferation assay was performed for detecting p53–MDM2 (a), EZH2–EED (b), PA7–Id1 (c), DIEDML–KIX (d), PB1N–PA (e), and PB2–PB1C (f) interactions with/without the serially diluted helper ligand (0.01, 0.1, 1, 10, 100 nM). The initial cell density was 1 × 105 cells/ml. The viable cell densities after 72 h are indicated as mean ± SD (n = 3, biological replicates).
