Figure 7
Determination of packaging strategy. (a) Genome coverage plot was implemented with the Bowtie2 software tool v.2.3.4.3 (https://cpt.tamu.edu/galaxy-pub). No peaks deviate significantly from the mean (yellow line). (b) Restriction analysis of Sam46 and Sam112 DNA with enzymes XbaI and HindIII; M—molecular weight markers; black arrows indicate pac-fragments. The full-length gel is presented in Supplementary Figure S5. (c) Location of the terminase-generated cut in the Sam46 genome. The terminal regions of the PCR product sequences obtained with RAGE for HindIII- and XbaI-generated pac-fragments are shown on the sequencing chromatograms.
