Figure 2

(A) Growth of primary tumors following treatment with nitrofen. Mice with established bilateral orthotopic 231-Luc tumors were administered the following agents by oral gavage on alternate days for 6 weeks, starting on day 12: olive oil (control) or nitrofen at 1, 3, or 6 mg/kg body weight as indicated. (B-E) Bioluminescence ‘heat map’ of metastases in the lungs and liver of tumor-bearing animals from this experiment. (B) untreated (control (fed with olive oil): Mouse 0–1 to 0–4); (C) Nitrofen at 6 mg/kg body Wt: Mouse 6–1 to 6–4; (D) Nitrofen at 3 mg/kg body Wt: Mouse 3–1 to 3–4; (E) Nitrofen at 1 mg/kg body Wt: Mouse 1–1 to 1–4. (F) Metastasis score determined by quantification of IVIS bioluminescence (shown in panels B–E) in the lungs and liver of untreated animals (control) or treated with various doses of nitrofen (1, 3, 6 mg/kg body Wt) every alternate day for 6 weeks. n = 5–10 tumor bearing mice per group. * significantly different compared to control lungs P < 0.05, # significantly different compared to control liver P < 0.05.