Figure 1
In (A–D) photomicrographs of mice tissues sections, evaluated in different enlargement in the microscopy, allowed us to observe how much the immunohistochemical technique by ADPH becomes essential, due to its sensitivity, to allow sebaceous differentiation in paraffin-embedded material, as demonstrated in the scheme represented in (E). In (F–H), we have photomicrographs of the sections of the mice’s frozen tissue stained with Sudan Black. Note the presence of black spots, provided by the labeling of the many lipids present in fresh tissue. This technique, when evaluated in a different objective, does not allow the sebaceous differentiation, as demonstrated in the scheme in (I).
