Figure 5

(a) Normalized absorption spectra of the A’α mutants. (b) λ_max in the D state and τ_1/2 values of dark regeneration with R_H values of the L212A and triple mutant in the D and L states. Elution profiles of the A’α mutants in the D (black lines) and L (blue lines) states, monitored at 280 nm at injected protein concentration of (c) 8 µM or (d) 50 µM. Black and blue dashed vertical lines indicate the peak volumes of wtPZ in the D and L states, respectively. The EC₅₀ values of the A’α mutants for dsApo according to EMSA (red bars) and QCM (green bars) in the (e) D and (f) L states with error bars (n ≥ 3). Numbers indicate EC₅₀ values and standard deviations (nM). *Data for wtPZ were taken from previous studies^15,16.